. 2020 Jan 16;98(1):skz377. doi: 10.1093/jas/skz377

Table 2.

Least squares means of relative expression of genes related to barrier function in duodenum and jejunum of broiler chickens fed diets with DON and mycotoxin deactivator (B) and with or without oral LPS challenge 1 d before sampling¹

	No LPS				LPS					P-values
Gene of interest	CON	CON-B	DON	DON-B	CON	CON-B	DON	DON-B	SEM	DON	B	LPS	DON × B	DON × LPS	B × LPS	DON × B × LPS
Duodenum
IAP	0.350	0.351	0.525	0.380	0.324	0.286	0.353	0.372	0.0498	0.027	0.252	0.058	0.530	0.530	0.378	0.153
MUC1	0.068	0.051	0.089	0.093	0.091	0.059	0.057	0.197	0.0368	0.112	0.359	0.327	0.068	0.700	0.252	0.150
MUC2	0.400	0.182	0.388	0.333	0.247	0.251	0.265	0.343	0.0757	0.247	0.376	0.357	0.270	0.893	0.101	0.681
CLDN1	0.425	0.361	0.530	0.433	0.448	0.427	0.391	0.524	0.0573	0.185	0.771	0.800	0.457	0.396	0.096	0.254
CLDN5	0.328	0.292	0.424	0.396	0.453	0.424	0.371	0.435	0.0827	0.581	0.901	0.306	0.667	0.250	0.674	0.717
OCLN	0.420	0.352	0.523	0.509	0.453	0.425	0.368	0.428	0.0513	0.224	0.729	0.367	0.330	0.021	0.436	0.824
ZO1	0.417	0.358	0.520	0.403	0.461	0.458	0.413	0.455	0.0571	0.547	0.399	0.583	0.931	0.222	0.186	0.531
Jejunum
IAP	0.350	0.409	0.382	0.409	0.324	0.323	0.444	0.340	0.0638	0.350	0.911	0.509	0.461	0.566	0.293	0.696
MUC1	0.399	0.180	0.259	0.266	0.336	0.301	0.244	0.210	0.0691	0.230	0.155	0.951	0.251	0.508	0.466	0.258
MUC2	0.464	0.430	0.412	0.415	0.257	0.245	0.340	0.305	0.0587	0.653	0.637	<0.001	0.935	0.208	0.921	0.718
CLDN1	0.708	0.731	0.765	0.721	0.781	0.765	0.706	0.688	0.0436	0.401	0.669	0.902	0.580	0.110	0.920	0.603
CLDN5	0.243	0.298	0.221	0.222	0.369	0.356	0.313	0.255	0.0692	0.199	0.935	0.119	0.614	0.765	0.520	0.961
OCLN	0.626	0.642	0.655	0.685	0.665	0.669	0.661	0.631	0.0377	0.777	0.860	0.873	0.854	0.289	0.515	0.654
ZO1	0.493	0.616	0.522	0.529	0.649	0.680	0.639	0.589	0.0474	0.242	0.406	0.004	0.145	0.753	0.273	0.793

¹CON, control diet. Values are least square means and pooled SEM; n = 10. Genes of interest: IAP, intestinal alkaline phosphatase;MUC1, mucin 1;MUC2, mucin 2;CLDN1, claudin1;CLDN5, claudin 5;OCLN, occludin;ZO1, zona occludens 1.