Table 3.

Least squares means of relative expression of genes related to toll-like receptor and cytokine signaling in duodenum and jejunum of broiler chickens fed diets with DON and mycotoxin deactivator (B) and with or without oral LPS challenge 1 day before sampling¹

	No LPS				LPS					P-values
Gene of interest	CON	CON-B	DON	DON-B	CON	CON-B	DON	DON-B	SEM	DON	B	LPS	DON × B	DON × LPS	B × LPS	DON × B × LPS
Duodenum
TLR2	0.370	0.354	0.508	0.414	0.379	0.350	0.360	0.333	0.0802	0.482	0.469	0.328	0.741	0.307	0.808	0.727
TLR4	0.363	0.317	0.493	0.394	0.478	0.414	0.378	0.467	0.0702	0.418	0.546	0.391	0.615	0.206	0.395	0.301
CAS3	0.436	0.326	0.496	0.449	0.410	0.371	0.336	0.460	0.0567	0.221	0.652	0.426	0.165	0.297	0.136	0.533
NFKB	0.413	0.339	0.537	0.478	0.509	0.413	0.423	0.481	0.0674	0.202	0.373	0.754	0.378	0.145	0.623	0.471
IL1B	0.140	0.198	0.275	0.201	0.254	0.273	0.287	0.256	0.0649	0.401	0.875	0.167	0.322	0.511	0.980	0.656
IL6	0.353	0.322	0.440	0.303	0.485	0.381	0.333	0.369	0.0649	0.606	0.206	0.421	0.850	0.214	0.587	0.189
IL8	0.147	0.164	0.260	0.187	0.297	0.249	0.186	0.236	0.0609	0.939	0.760	0.230	0.959	0.135	0.734	0.279
IL10	0.164	0.062	0.095	0.330	0.205	0.132	0.196	0.526	0.1299	0.119	0.297	0.274	0.050	0.616	0.739	0.857
TGF1B	0.297	0.282	0.402	0.386	0.318	0.322	0.290	0.437	0.0645	0.109	0.514	0.994	0.435	0.505	0.321	0.436
TNFA	0.477	0.345	0.569	0.515	0.428	0.406	0.352	0.407	0.0589	0.267	0.362	0.065	0.357	0.048	0.195	0.994
Jejunum
TLR2	0.294	0.346	0.344	0.310	0.408	0.342	0.324	0.228	0.0796	0.413	0.527	0.971	0.607	0.348	0.424	0.806
TLR4	0.348	0.373	0.282	0.344	0.516	0.357	0.449	0.378	0.0682	0.468	0.460	0.071	0.528	0.802	0.105	0.792
CAS3	0.517	0.555	0.471	0.542	0.495	0.529	0.550	0.485	0.0447	0.710	0.541	0.828	0.602	0.582	0.267	0.301
NFKB	0.440	0.464	0.330	0.445	0.622	0.369	0.503	0.495	0.0597	0.464	0.473	0.070	0.050	0.424	0.021	0.365
IL1B	0.366	0.377	0.382	0.405	0.507	0.450	0.492	0.327	0.0885	0.703	0.456	0.329	0.703	0.474	0.310	0.635
IL6	0.286	0.348	0.313	0.371	0.509	0.405	0.422	0.331	0.0533	0.463	0.621	0.023	0.954	0.170	0.041	0.916
IL8	0.334	0.220	0.218	0.262	0.294	0.215	0.282	0.201	0.0711	0.622	0.256	0.838	0.443	0.812	0.655	0.429
IL10	0.325	0.064	0.238	0.224	0.236	0.127	0.204	0.232	0.0750	0.495	0.099	0.805	0.076	0.998	0.367	0.605
TGF1B	0.378	0.354	0.439	0.451	0.402	0.429	0.397	0.344	0.0625	0.700	0.832	0.777	0.808	0.164	0.935	0.515
TNFA	0.786	0.778	0.825	0.796	0.711	0.784	0.747	0.776	0.0350	0.406	0.514	0.096	0.519	0.766	0.162	0.817

¹CON, control diet. Values are least square means and pooled SEM; n = 10. Genes of interest: TLR2, toll-like receptor 2;TLR4, toll-like receptor 4;CAS, caspase 3;NFKB, nuclear factor kappa B;IL1B, interleukin 1s; IL6, interleukin 6;IL8, interleukin 8;IL10, interleukin 10;TGFB1, transforming growth factor beta 1;TNFA, lipopolysaccharide-induced tumor necrosis factor-alpha.