Skip to main content
. 2020 Jan 16;98(1):skz377. doi: 10.1093/jas/skz377

Table 5.

Least squares means of α-diversity and relative bacterial abundance of selected bacterial taxa in cecal digesta of broiler chickens fed diets with DON and mycotoxin deactivator (B) and with or without oral LPS challenge 1 d before sampling1

No LPS LPS P-values
Item2,3 CON CON-B DON DON-B CON CON-B DON DON-B SEM DON B LPS DON × B DON × LPS B × LPS DON × B × LPS
α-diversity metrices
Shannon 6.15 6.43 6.24 6.30 6.34 6.40 6.18 6.18 0.118 0.217 0.236 0.960 0.418 0.297 0.387 0.646
Simpson 0.94 0.96 0.95 0.95 0.96 0.96 0.95 0.95 0.012 0.648 0.248 0.331 0.697 0.712 0.378 0.733
Chao1 1517 1417 1384 1488 1411 1450 1345 1389 67.527 0.325 0.654 0.272 0.278 0.730 0.684 0.303
Family
f_Lachnospiraceae 31.7 34.8 32.1 29.2 33.5 31.6 29.7 33.1 1.598 0.102 0.715 0.985 0.878 0.495 0.770 0.015
f_Ruminococcaceae 16.9 17.3 14.3 16.2 17.7 16.4 15.2 14.2 0.866 0.001 0.942 0.616 0.466 0.648 0.066 0.668
f_Bacteroidaceae 13.0 6.0 12.8 10.5 8.8 10.7 10.4 9.3 1.997 0.427 0.138 0.570 0.763 0.489 0.081 0.171
f_Lactobacillaceae 5.9 6.7 5.5 6.5 4.5 5.2 7.8 7.4 1.118 0.127 0.493 0.939 0.768 0.065 0.638 0.666
f_Turicibacteraceae 0.53 0.73 0.51 0.34 0.85 0.31 0.58 0.63 0.181 0.486 0.362 0.609 0.654 0.369 0.315 0.063
f_Coriobacteriaceae 0.23 0.28 0.17 0.21 0.26 0.22 0.19 0.24 0.031 0.064 0.292 0.706 0.389 0.365 0.402 0.208
f_Bifidobacteriaceae 0.094 0.18 0.072 0.067 0.30 0.31 0.13 0.13 0.088 0.056 0.712 0.066 0.698 0.375 0.800 0.764
f_Peptostreptococcaceae 0.064 0.084 0.064 0.083 0.095 0.075 0.11 0.070 0.022 0.833 0.706 0.343 0.688 0.811 0.096 0.717
f_[Mogibacteriaceae] 0.049 0.037 0.042 0.033 0.039 0.042 0.037 0.038 0.005 0.176 0.219 0.716 0.953 0.759 0.071 0.725
f_Leuconostocaceae 0.009 0.058 0.037 0.017 0.031 0.032 0.033 0.035 0.012 0.811 0.363 0.762 0.057 0.597 0.449 0.042
o_RF39 0.020 0.024 0.009 0.018 0.012 0.043 0.002 0.008 0.013 0.096 0.179 0.873 0.591 0.441 0.567 0.423
Genus
g_[Ruminococcus] 12.8 16.2 13.5 11.8 14.7 12.8 11.5 13.2 1.167 0.050 0.637 0.513 0.630 0.797 0.548 0.011
g_Bacteroides 13.0 6.0 12.8 10.5 8.8 10.7 10.4 9.3 1.997 0.427 0.138 0.570 0.763 0.489 0.081 0.171
f_Ruminococcaceae1 7.8 8.3 6.3 8.1 9.0 8.2 6.6 6.7 0.646 0.003 0.368 0.998 0.260 0.236 0.113 0.838
g_Lactobacillus 5.9 6.7 5.5 6.5 4.5 5.2 7.8 7.4 1.118 0.127 0.493 0.939 0.768 0.065 0.638 0.666
g_Oscillospira 5.2 5.1 4.6 4.9 5.0 5.0 5.0 4.1 0.317 0.054 0.390 0.458 0.644 0.844 0.204 0.135
g_Ruminococcus 3.6 3.8 3.2 3.0 3.4 3.0 3.4 3.3 0.270 0.214 0.503 0.507 0.909 0.068 0.462 0.405
g_Dorea 1.3 1.7 1.4 1.3 1.7 1.7 1.2 1.1 0.191 0.013 0.930 0.861 0.248 0.134 0.380 0.547
f_Lachnospiraceae2 0.70 0.62 0.68 0.57 0.59 0.55 0.48 0.64 0.058 0.567 0.736 0.062 0.333 0.818 0.061 0.184
g_Turicibacter 0.53 0.73 0.51 0.34 0.85 0.31 0.58 0.63 0.181 0.486 0.362 0.609 0.654 0.369 0.315 0.063
g_Coprobacillus 0.42 0.36 0.59 0.50 0.40 0.44 0.50 0.52 0.085 0.042 0.715 0.966 0.816 0.636 0.414 0.991
g_Clostridium 0.39 0.20 0.41 0.20 0.25 0.11 0.24 0.22 0.110 0.715 0.073 0.227 0.756 0.811 0.442 0.684
g_Eggerthella 0.17 0.22 0.13 0.16 0.20 0.15 0.15 0.19 0.027 0.097 0.392 0.883 0.383 0.254 0.245 0.124
g_Bifidobacterium 0.094 0.18 0.072 0.067 0.30 0.31 0.13 0.13 0.088 0.056 0.712 0.066 0.698 0.375 0.800 0.764
f_Erysipelotrichaceae1 0.12 0.19 0.16 0.049 0.17 0.12 0.070 0.098 0.049 0.143 0.648 0.654 0.429 0.837 0.854 0.065
f_Peptostreptococcaceae1 0.063 0.084 0.063 0.083 0.094 0.074 0.11 0.069 0.022 0.827 0.713 0.353 0.682 0.808 0.099 0.711
f_Ruminococcaceae2 0.097 0.090 0.068 0.091 0.086 0.065 0.079 0.052 0.011 0.131 0.311 0.042 0.444 0.825 0.044 0.260
f_[Mogibacteriaceae] 1 0.049 0.037 0.042 0.033 0.039 0.042 0.037 0.038 0.005 0.176 0.219 0.716 0.953 0.759 0.071 0.725
f_Leuconostocaceae1 0.009 0.058 0.037 0.017 0.031 0.032 0.033 0.035 0.012 0.811 0.363 0.762 0.057 0.597 0.449 0.042
o_RF391 0.020 0.024 0.009 0.018 0.012 0.043 0.002 0.008 0.013 0.096 0.179 0.873 0.591 0.441 0.567 0.423

1CON, control diet. Values are least square means and pooled SEM; n = 10.

2Rarefaction depth of 10,000 sequences to determine α-diversity.

3Differently abundant bacterial families and genera with a relative abundance >0.01%.