. Author manuscript; available in PMC: 2020 Jan 28.

Published in final edited form as: Anal Biochem. 1992 May 1;202(2):316–330. doi: 10.1016/0003-2697(92)90112-k

TABLE 4.

Phase, Modulation, and Lifetime of Y_t-base with Acrylamide^a

[Ac] (m)	Method^b	Phase		Modulation
[Ac] (m)	Method^b	θ (degrees)	τ_θ (ns)^c	m	τ_m (ns)^c
0.0	FD	65.5	4.38	0.394	4.65
	Cosine^d	⟨65.5⟩ ± 3.4	4.38 ± 0.71	⟨0.394⟩ ± 0.02	4.65 ± 0.28
	CCDFT^d	⟨65.5⟩ ± 3.0	4.35 ± 0.45	⟨0.394⟩ ± 0.03	4.64 ± 0.45
0.1	FD	46.1	2.08	0.668	2.22
	Cosine	47.1 ± 2.3	2.14 ± 0.17	0.720 ± 0.03	1.92 ± 0.17
	CCDFT	46.7 ± 2.0	2.10 ± 0.15	0.699 ± 0.04	2.06 ± 0.30
0.2	FD	34.3	1.36	0.8	1.5
	Cosine	34.6 ± 2.0	1.37 ± 0.10	0.858 ± 0.03	1.19 ± 0.16
	CCDFT	33.1 ± 2.0	1.29 ± 0.20	0.844 ± 0.04	1.26 ± 0.30
0.4	FD	22.3	0.82	0.884	1.06
	Cosine	24.6 ± 3.4	0.91 ± 0.15	0.892 ± 0.04	1.01 ± 0.22
	CCDFT	23.5 ± 2.0	0.85 ± 0.10	0.902 ± 0.04	0.94 ± 0.30

Excitation wavelength, 345 nm; Corning 3-75 emission filter; near 25°C (room temperature).

FD, standard frequency-domain measurements at 79.695 MHz; Cosine, fit to Eq. [1] at 80.01 MHz; CCDFT, pixel-by-pixel fit using the algorithm in Appendix 1 at 80.01 MHz.

See Table 1, footnote c.

The angle brackets indicate that these values were used as reference values.