Table 5.
Effect of dietary pterostilbene supplementation on the expression levels of jejunal genes related to inflammation and tight junction of lipopolysaccharide-challenged broilers1
Items2 | CON-SS | PT-SS | CON-LPS | PT-LPS | P-value | ||
---|---|---|---|---|---|---|---|
LPS | Diet | L × D | |||||
TLR4 | 1.00 ± 0.36 | 0.68 ± 0.14 | 1.58 ± 0.24 | 0.92 ± 0.19 | 0.117 | 0.064 | 0.500 |
IL-1β | 1.00 ± 0.18 | 0.67 ± 0.11 | 1.62 ± 0.19 | 0.79 ± 0.18 | 0.042 | 0.003 | 0.148 |
IL-10 | 1.00 ± 0.13 | 1.18 ± 0.21 | 1.01 ± 0.17 | 1.09 ± 0.17 | 0.823 | 0.453 | 0.767 |
NLRP3 | 1.00 ± 0.16 | 0.84 ± 0.15 | 4.01 ± 1.30 | 1.25 ± 0.23 | 0.019 | 0.041 | 0.067 |
ZO-1 | 1.00 ± 0.13 | 1.38 ± 0.22 | 0.56 ± 0.08 | 0.83 ± 0.08 | 0.002 | 0.031 | 0.692 |
OCLN | 1.00 ± 0.10 | 1.26 ± 0.11 | 0.79 ± 0.16 | 1.16 ± 0.13 | 0.241 | 0.024 | 0.676 |
1Values are means ± SE, n = 6
2CON-LPS, broilers fed a basal diet and an injection of lipopolysaccharide; CON-SS, broilers fed a basal diet and an injection of sterile saline; L × D = the interaction effect of main effects (LPS and Diet); LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor protein 3; OCLN, occludin; PT-LPS, broilers fed a basal diet supplemented with 400 mg/kg pterostilbene and an injection of lipopolysaccharide; PT-SS, broilers fed a basal diet supplemented with 400 mg/kg pterostilbene and an injection of sterile saline; TLR4, toll-like receptor 4; ZO-1, tight junction protein 1.