Table 1.
Ingredient composition of experimental diets for phase 1
| Item, % | Positive control | Negative control, (NC1) | NC+ 1,250 cfu | NC + 2,500 cfu | NC + 3,500 cfu |
|---|---|---|---|---|---|
| Corn | 45.35 | 46.35 | 46.25 | 46.15 | 46.07 |
| Whey, dried | 15.00 | 15.00 | 15.00 | 15.00 | 15.00 |
| SBM, 48% | 28.00 | 28.0 | 28.0 | 28.0 | 28.0 |
| Fish meal | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Choice white grease | 3.00 | 3.00 | 3.00 | 3.00 | 3.00 |
| Limestone | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Dicalcium phosphate | 0.35 | 0.35 | 0.35 | 0.35 | 0.35 |
| l-Lys HCl | 0.37 | 0.37 | 0.37 | 0.37 | 0.37 |
| dl-Met | 0.14 | 0.14 | 0.14 | 0.14 | 0.14 |
| l-Thr | 0.09 | 0.09 | 0.09 | 0.09 | 0.09 |
| Salt | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 |
| Vitamin–mineral premix2 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 |
| Mecadox premix3 | 1.00 | — | — | — | — |
| C. butyricum premix4 | — | — | 0.10 | 0.20 | 0.28 |
1NC, negative control.
2Provided the following quantities of vitamins and micro-minerals per kilogram of complete diet: Vitamin A as retinyl acetate, 11,136 IU; vitamin D3 as cholecalciferol, 2,208 IU; vitamin E as dl-alpha tocopheryl acetate, 66 IU; vitamin K as menadione dimethylprimidinol bisulfite, 1.42 mg; thiamin as thiamine mononitrate, 0.24 mg; riboflavin, 6.59 mg; pyridoxine as pyridoxine hydrochloride, 0.24 mg; vitamin B12, 0.03 mg; d-pantothenic acid as d-calcium pantothenate, 23.5 mg; niacin, 44.1 mg; folic acid, 1.59 mg; biotin, 0.44 mg; Cu, 20 mg as copper sulfate and copper chloride; Fe, 126 mg as ferrous sulfate; I, 1.26 mg as ethylenediamine dihydriodide; Mn, 60.2 mg as manganese sulfate; Se, 0.3 mg as sodium selenite and selenium yeast; and Zn, 125.1 mg as zinc sulfate.
3The Mecadox premix provided 50 mg/kg of Carbadox to the complete diet.
4The C. butyricum premix was added to provide 1,250 × 108 cfu/kg of complete diet, 2,500 108 cfu/kg of complete diet, or 3,500 × 108 cfu/kg of complete diet.