Table 1.
List of primers and probes
| Gene name | Species | Application, Chemistry | Company | Sequence/Catalogue number |
|---|---|---|---|---|
| ATG16L1 HM | Mouse | Genotyping | IDT | P1: TGGCTGGAGTGCGATCTTCC P2: CAGACGGCAAACGACTGTCCT P3: CAGGATCCTTCTGCACACATTT P4: CACCTGGTTACATTGGCAAACA |
| PR Cre | Mouse | Genotyping | IDT | P1: ATG TTT AGC TGG CCC AAA TG P2: TAT ACC GAT CTC CCT GGA CG P3: CCC AAA GAG ACA CCA GGA AG |
| Atg16L1 flox | Mouse | Genotyping | IDT | P1: GGAACCACGCTGACATTTGACACTG P2: CAAAGAACAACGAGTGGCAGTAG P3: CATCAGATACACTAGAGCTGG |
| Prp | Mouse | qPCR, Sybr | IDT | F: TCC TGG CCA ATA ATG CTG CCA TTG |
| R: AGC AGC CAT TCT CTC CTG TTT GAC | ||||
| 18S | Mouse | qPCR, Sybr | IDT | F: TTC CTT ACC TGG TTG ATC CTG CCA |
| R: AGC CAT TCG CAG TTT CAC TGT ACC | ||||
| Wnt4 | Mouse | qPCR, Taqman | ABI | Mm01194003_m1 |
| Bmp2 | Mouse | qPCR, Taqman | ABI | Mm01340178_m1 |
| Atg16L1 | Mouse | qPCR, Taqman | ABI | Mm00513084_m1 |
| 18S | Mouse | qPCR, Taqman | ABI | 4318839 |
| IGFBP1 | Human | qPCR, Taqman | ABI | Hs00236877_m1 |
| PRL | Human | qPCR, Taqman | ABI | Hs00168730_m1 |
All primer sequences are written 5’ to 3’
Abbreviations: ABI, Applied Biosystems; IDT, integrated DNA technologies; qPCR, quantitative polymerase chain reaction.