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. 2019 Nov 21;161(1):bqz021. doi: 10.1210/endocr/bqz021

Figure 1.

Figure 1.

IRS2 is expressed in HESC during decidualization. IRS2 mRNA (A) and protein (B) expression was observed in HESC treated with DC (E+P+cAMP) for 0 to 6 days. (A) Gene expression analysis was performed using primers specific for IRS2. 36B4 was used for normalization. Data are represented as the mean fold induction ± SEM. *P < 0.05 relative to day 0. (B) Whole cell lysates were analyzed by Western blot and probed with antibody specific to IRS2. Calnexin is provided as a loading control. Densitometry analysis is provided (below). (C) Following siRNA-mediated knockdown of IRS2 and stimulation with DC for 72 hours, gene expression analysis was performed using primers specific to IRS1 and IRS2. 36B4 was used for normalization. Data are represented as the mean fold induction ± SEM. *P < 0.05 relative to siCtrl. (D) Following knockdown and stimulation with DC for 72 hours, IRS2 expression was determined by Western blot. Calnexin is provided as a loading control. Densitometry analysis is provided (below).

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