Figure 7.

Loss of IRS2 reduces glucose uptake by HESC undergoing decidualization. (A) HESC were treated for 72h with vehicle (Veh; ethanol) or DC following siRNA mediated knockdown of IRS2. Cells were washed with PBS and incubated with 50μM of 2-NBDG in glucose-free media for 1 hour. Cells were then washed and 2-NBDG incorporation was measured and normalized to total protein. Data are represented as the mean fold glucose uptake ± SEM. *P < 0.05 relative to siCtrl treated with vehicle. (B) HESC were stimulated with DC in the presence of full (100%; 17.5mM) or reduced (75% and 50%; 13 and 8.75mM, respectively) glucose concentrations in the culture media for 72 hours. Gene expression analysis was performed using primers specific for IGFBP1 and WNT4. 36B4 was used for normalization. Data are represented as the mean fold induction ± SEM. *P < 0.05 relative to DC in full glucose concentration of the culture media.