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. 2019 Jul 15;18(17):2110–2123. doi: 10.1080/15384101.2019.1641388

Figure 2.

Figure 2.

SIRT1 enhanced the expression of miR17–92 cluster through stabilization of C-Myc. (a) Schematic diagram of the two promoter regions of miR17–92 cluster. (b) Determination of luciferase activity. Cells were co-transfected with Promoter1 or Promoter2 with siNC or siSIRT1. Columns, mean of at least three independent experiments; bars, SEM. *, P < 0.05, comparison between two groups as indicated. (c) Determination of luciferase activity. Cells were co-transfected with Promoter1 or Promoter2 with pReceiver-M02 or SIRT1-overexpression plasmid (SIRT1). Columns, mean of at least three independent experiments; bars, SEM. *, P < 0.05, comparison between two groups as indicated. (d) Western blot was carried out to monitor the expression levels of SIRT1, C-Myc and Acetyl-H4K16 in cells transfected with siNC/siSIRT1, or pReceiver-M02/SIRT1-overexpression plasmid (SIRT1). (e) Real-time qPCR was used to detect the expression levels of C-Myc mRNA in 293 cells transfected with siNC or siC-Myc-1/2/3. Columns, mean of at least three independent experiments; bars, SEM. **, P < 0.01, comparison between two groups as indicated. (f) Western blot was performed to evaluate the expression levels of C-Myc in cells transfected with siNC or siC-Myc-1/2/3. (g) Expression of miR17HG and miR-17-92 miRNAs in siNC or siC-Myc transfected cells. Columns, mean of at least three independent experiments; bars, SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001, comparison between two groups as indicated. (h) Expression of miR17HG and miR-17-92 miRNAs in cells cotransfected with siNC/siSIRT1, or pReceiver-M02/SIRT1-overexpression plasmid (SIRT1). Columns, mean of at least three independent experiments; bars, SEM. *, P < 0.05; **, P < 0.01, comparison between two groups as indicated.