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The positive feedback loop between miR17HG and SIRT1. (a) Western blot was performed to monitor the expression levels of SIRT1 and Acetyl-H4K16 in cells transfected with siNC, simiR17HG1-1/2, pcDNA3.1, or miR17HG. (b) RIP-coupled PCR assay of the interaction between SIRT1 and miR17HG in 293 cells. (c) Schematic diagram of the positive feedback loop of SIRT1 and miR17HG, and the effect of this loop in DSBs repair.
