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. 2020 Jan 23;15:483–495. doi: 10.2147/IJN.S229858

Figure 6.

Figure 6

Flow cytometry and Western blot analysis of EGFRvIII-CAR expressed in pEGFRvIII-CAR@SNPG1/800-transfected Jurkat cells. (A). Structural diagram of EGFRvIII-CAR. (B). Protein L-based flow cytometry analysis of Jurkat cells transfected w/or w/o pEGFRvIII-CAR@SNPsG1/800. (C). Western blot analysis of EGFRvIII-CAR expressed in pEGFRvIII-CAR@SNPsG1/800-transfected Jurkat cells.

Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; EGFRvIII, epidermal growth factor receptor variant III; CAR, chimeric antigen receptor; pEGFRvIII-CAR, epidermal growth factor receptor variant III-chimeric antigen receptor expression plasmid; SNPs, self-assembled nanoparticles.