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. 2020 Jan 23;15:483–495. doi: 10.2147/IJN.S229858

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© 2020 Yu et al.

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Interaction of EGFRvIII-CAR-positive Jurkat T cells with EGFRvIII-positive HuH7 cells. (A). Scheme of the interaction of EGFRvIII-CAR-positive Jurkat T cell with EGFRvIII-positive HuH7 cell. (B). Western blot analysis of EGFRvIII expressed in HuH7 engineered to stably overexpress EGFRvIII. (C). Confocal microscopy-based studies on the interaction between EGFRvIII-positive HuH7 cells (green) and Jurkat T cells (red) transfected w/or w/o pEGFRvIII-CAR@SNPs^G1/800. Scale bars, 20 µm.

Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; EGFR, epidermal growth factor receptor; EGFRvIII, epidermal growth factor receptor variant III; CSFE, carboxyfluorescein succinimidyl ester; Dil, 1,1′-Dioetadeeyl-3,3,3′,3′-tetramethylindocarboeyanine perchlorate.