Table 1. Overview, in chronological order, of the data collection in ranch horses during the pre- and post-workday assessments.

Period		Time	Data collection	Assessments
Pre-workday assessments	Day before	Between 17:30 and 18:30 h	Clinical examination	Body condition score and lameness assessment
			Blood sample	Total protein, cortisol, lactate, glucose, creatine kinase and aspartate aminotransferase
	Before the start of the routine workday	Between 06:30 and 08:30 h, i.e. 40–50 min before workday	Clinical examination	Lameness assessment
			Respiratory rate—Direct observation	Counting flank/nostril movements for 15 s
			Other behaviours—video record	5 min filming of horse’s body
			Face expressions—video record	5 min filming of horse’s face
Post-workday assessments	Immediately after work	At the end of the workday	Clinical examination	Lameness assessment
		Immediately after lameness assessment post-workday	Respiratory rate—direct observation	Counting flank/nostril movements for 15 s
		Immediately post-workday	Blood sample	Total protein, cortisol, lactate, glucose, creatine kinase and aspartate aminotransferase
		3 min post-workday	Other behaviours—video record	5 min filming of horse’s body
		10–12 min post-workday	Face expressions—video record	5 min filming of horse’s face
		15 min post-workday	Blood sample	Lactate and glucose
		30 min post-workday	Blood sample	Lactate and glucose
		60 min post-workday	Blood sample	Lactate and glucose
		75 min post-workday	Clinical examination	Lameness assessment
	Later after work	6 h post-workday	Blood sample	Creatine kinase and aspartate aminotransferase
		12 h post-workday	Blood sample	Creatine kinase and aspartate aminotransferase