Fig 3. Experimental protocol for repeated dosing study using MC4R KO mice.

Eleven-week-old male MC4R KO mice were fed with WD for 22 weeks. Drug treatments were started 13 weeks after pre-feeding. GS-0976 (4 and 16 mg/kg/day) was orally administered twice a day for 9 weeks starting at 24 weeks of age. The white triangle marks the collection of blood before and after the 8-week treatment. The black triangle marks harvesting of the liver after 9 weeks of treatment.