Table 2. Effects of GS-0976 on steatosis, lobular inflammation, ballooning degeneration, and NAFLD activity score.

Mice	Control	MC4R KO
Treatment	Vehicle	Vehicle	GS-0976
Dose (mg/kg/day)			4	16
	(n = 5)	(n = 8)	(n = 8)	(n = 8)
Steatosis	0.0 ± 0.0	3.0 ± 0.0	1.9 ± 0.4	1.3 ± 0.5
Lobular inflammation	0.0 ± 0.0	1.0 ± 0.0	0.9 ± 0.4	1.0 ± 0.0
Ballooning degeneration	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0
NAFLD activity score	0.0 ± 0.0	4.0 ± 0.0	2.8 ± 0.7	2.3 ± 0.5

Individual scores for steatosis (0–3), inflammation (0–3) and ballooning (0–2) were provided and were added up to determine the NAFLD activity score as a semi-quantitative measure of disease severity. Steatosis, inflammation and hepatocyte ballooning were scored according to the previous report [33].