Figure 3.

Let-7c and Wnt signaling inhibitor blocks the symmetric division.

Wnt activation inhibitor of Axitinib was used as positive control. (a) Both Axitinib and Let-7c effectively promoted the asymmetric division (above), suppressed the symmetric division (below). The differences of ACD ratios among groups of Let-7c, Let-7b, and Axitinib in T47-D (F = 0.223, p = 0.801), MDA-MB-231 (F = 2.066, p = 0.136) and BT-20 (F = 0.526, p = 0.594) are not significant, and similarly, the differences of SCD ratios among groups of Let-7c, Let-7b, and Axitinib in T47-D (F = 0.584, p = 0.561), MDA-MB-231 (F = 2.159, p = 0.126) and BT-20 (F = 0.253, p = 0.778) are not significant, processing by ANOVA analysis. (b) Images of fluorescence results of EdU dye were shown, and the dividing cells were marked with either the white arrow (SCD) or the yellow arrow (ACD). (c) Axitinib and Let-7c decreased number of the forming spheres significantly (left), which was directly attributed to the self-renewal ability of stem cells, with representative images shown in right. ACD, asymmetric division, SCD, symmetric division. Results were acquired and calculated from six independent experiments.