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(A) Tm values obtained from tetraplex and M. mycetomatis-specific qPCR assays for M. fahalii, M. mycetomatis, M. pseudomycetomatis, and M. tropicana. (B) Tetraplex and MM-specific qPCR with a blinded test set and DNA samples obtained from patients yielded 100% specificity and sensitivity if the threshold cycles <30 (red line).
