Figure 5. Founder granule disassembly follows oskar degradation.

(A) Quantification of total fluorescence intensity of oskar in the germ plasm from nc2 to nc12 normalized to the average intensity at nc2; n = 5–13 embryos for each nc. (B) Quantification of the average number of mRNAs per detected oskar particle from nc2 to nc12; n = 4–10 embryos for each nc. (C) Quantification of the average fluorescence intensity (size) of Staufen particles in the germ plasm from nc3 to nc12; n = 3–8 embryos for each nc. (D) Quantification of the proportion of detected Staufen particles colocalized with oskar from nc2 to nc12; n = 4–10 embryos for each nc. (E) Quantification of the proportion of detected oskar particles colocalized with Staufen from nc2 to nc12; n = 4–10 embryos for each nc. (F) Ratio of the average fluorescence intensities for 5' and 3' oskar probes measured particles of the indicated sizes in nc3-4 versus nc7-8 embryos (n = 10 embryos each time period). (G,H) Proportion of oskar particles in the germ plasm containing the indicated number of mRNAs that are colocalized with DCP1 (G) and proportion of all oskar particles in the germ plasm with the indicated number of mRNA (H); n = 6–9 embryos for each nc. Values in (A), (D), and (E) are mean ± s.d; values in (B,C,F) are mean ± s.e.m. Student's t-tests were performed comparing each nuclear cycle to nc2 (A,B,D,E), nc3 (C), and nc3-4 (F); *p<0.05, **p<0.01, ***p<0.001 as determined by Student's t-test. Arrowheads indicates time of pole cell formation.