Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Ambystoma mexicanum) | cagg:egfp | Ambystoma Genetic Stock Center (Sobkow et al., 2006) |
AGSC Cat# 110A, RRID:AGSC_110A | |
| Genetic reagent (Ambystoma mexicanum) | cagg:nls-mcherry | Ambystoma Genetic Stock Center (Kragl et al., 2009) |
AGSC Cat# 112A, RRID:AGSC_112A | |
| Chemical compound, drug | MS-222 | Western Chemical | ANADA #200–226 | |
| Chemical compound, drug | Human chorionic gonadotropin (Chorulon,) | Merck Animal Health | NADA 140–927 | |
| Gene (Ambystoma mexicanum) | msx2 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000067092 | |
| Gene (Ambystoma mexicanum) | prmt1 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000062704 | |
| Gene (Ambystoma mexicanum) | myl6 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000067862 | |
| Gene (Ambystoma mexicanum) | fetub | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000227254 | |
| Gene (Ambystoma mexicanum) | hoxc8 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000065333 | |
| Gene (Ambystoma mexicanum) | akap8l | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000192860 | |
| Gene (Ambystoma mexicanum) | hrnrpa0 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000081837 | |
| Gene (Ambystoma mexicanum) | hsd17b10 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000257015 | |
| Gene (Ambystoma mexicanum) |
hoxb9 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000035333 | |
| Gene (Ambystoma mexicanum) | tyrosinase | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000179254 | |
| Gene (Ambystoma mexicanum) | etv4 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000233035 | |
| Gene (Ambystoma mexicanum) | cacng1 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000081988 | |
| Gene (Ambystoma mexicanum) | catalase | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000186723 | |
| Gene (Ambystoma mexicanum) | hoxb13 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000007929 | |
| Gene (Ambystoma mexicanum) | zic5 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000057641 | |
| Gene (Ambystoma mexicanum) | ecm1 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000123229 | |
| Gene (Ambystoma mexicanum) | cornifelin | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000173184 | |
| Gene (Ambystoma mexicanum) | dsg-like | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000056512 | |
| Gene (Ambystoma mexicanum) | enpp2 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000217071 | |
| Gene (Ambystoma mexicanum) | fabp2 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000084459 | |
| Gene (Ambystoma mexicanum) | pmp2 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000238807 | |
| Gene (Ambystoma mexicanum) | kcne1 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000121776 | |
| Gene (Ambystoma mexicanum) | krt6a | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000060835 | |
| Gene (Ambystoma mexicanum) | rcc1 | Axolotl transcriptome assembly 3.4 | AMEXTC_0340000210022 | |
| Recombinant DNA reagent | MLM3613 | (Hwang et al., 2013) | RRID: Addgene plasmid 42251 | Cas9 expression vector |
| Peptide, recombinant protein | Cas9 | PNABio | Cat. #: CP04-500 | |
| Commercial assay or kit | mMessage mMachine Kit | ThermoFisher | Cat. #: Am1345 | |
| Commercial assay or kit | MAXIscript SP6/T7 Transcription Kit | ThermoFisher | Cat. #: Am1322 | |
| Chemical compound, drug | MS-222 | Sigma Aldrich | SML1656 | |
| Software, algorithm | Geneious Software | Biomatters | RRID:SCR_010519 |