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. Author manuscript; available in PMC: 2020 Jan 28.

Published in final edited form as: Am J Hematol. 2019 Jan 3;94(3):327–337. doi: 10.1002/ajh.25384

Figure 2. — Dorsal skin-fold chambers were implanted onto SS mice (n=3/group) and AA mice (n=3/group) and 20 – 24 flowing venules were selected in each mouse at baseline (time 0). (A) SS mice were infused with 100 μl of zymosan-activated C57 mouse serum or zymosan-treated heat-inactivated C57 mouse serum at baseline after selection of flowing venules. Microvascular stasis (% non-flowing venules) was measured in the same venules at 1 and 4 hours after infusion. Bars represent means ± SD. *P<.05 and ***P<.001 versus heat-inactivated serum. (B) AA and SS-mice (n=3/group) were infused with 10 ng of C5a. Microvascular stasis was measured 1, 2, 3 and 4 h after infusion. Values are means ± SD. *P≤.05 and **P≤.01 versus AA mice. (C) SS mice (n=3/group) were infused with 200 ng of C5a. One group of SS mice was pre-treated with 30 μg of anti-C5a receptor (C5aR) IgG 30 minutes prior to C5a infusion. Microvascular stasis was measured 1, 2, 3 and 4 hours after C5a infusion. Values are means ± SD. **P≤0.01 and ***P≤0.001 versus C5a. Comparison of treatment groups at each time point was made using an unpaired t-test.

Figure 2. — Dorsal skin-fold chambers were implanted onto SS mice (n=3/group) and AA mice (n=3/group) and 20 – 24 flowing venules were selected in each mouse at baseline (time 0). (A) SS mice were infused with 100 μl of zymosan-activated C57 mouse serum or zymosan-treated heat-inactivated C57 mouse serum at baseline after selection of flowing venules. Microvascular stasis (% non-flowing venules) was measured in the same venules at 1 and 4 hours after infusion. Bars represent means ± SD. *P<.05 and ***P<.001 versus heat-inactivated serum. (B) AA and SS-mice (n=3/group) were infused with 10 ng of C5a. Microvascular stasis was measured 1, 2, 3 and 4 h after infusion. Values are means ± SD. *P≤.05 and **P≤.01 versus AA mice. (C) SS mice (n=3/group) were infused with 200 ng of C5a. One group of SS mice was pre-treated with 30 μg of anti-C5a receptor (C5aR) IgG 30 minutes prior to C5a infusion. Microvascular stasis was measured 1, 2, 3 and 4 hours after C5a infusion. Values are means ± SD. **P≤0.01 and ***P≤0.001 versus C5a. Comparison of treatment groups at each time point was made using an unpaired t-test.