Figure 3. DMXAA induces a transient regression of TP melanomas but not of isogenic SP tumors.

(A) B10.D2 mice were injected subcutaneously with 2 million T429.11 cells, a cell line derived from an induced Amela TiRP tumor (Zhu et al., 2017) (TP tumors). (B) TiRP mice were treated subcutaneously with 2 mg of 4-OH tamoxifen to induce tumor development as described in Zhu et al. (2017) (SP tumors). Tumor growth was measured with calipers and approximated to the volume of a spheroid (d².D/2). When the average diameter was between 7 and 9 mm, T429-tumor-bearing mice or TiRP-tumor-bearing mice were randomized into two groups, with an average tumor volume of 220 mm³, and were treated with a single intraperitoneal (i.p.) injection of DMXAA (23 mg/kg) or DMSO (day 0). Tumor growth was monitored. Graphs show means ± S.E.M.