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. Author manuscript; available in PMC: 2020 Jan 28.
Published in final edited form as: J Allergy Clin Immunol. 2018 Jun 18;143(3):1143–1152.e4. doi: 10.1016/j.jaci.2018.06.005

Figure 1. Survival and key antigen-presentation molecules of activated neutrophils from allergic individuals.

Figure 1

(A) Neutrophils from six birch pollen-allergic subjects were stimulated with GM-CSF/IFN-γ/IL-3 (gray) or cultured in medium alone (white box plots). Vital and Annexin-V-positive cells were assessed at the indicated time points by flow cytometry; (B) Neutrophils or monocytes from seven birch pollen-allergic subjects were stimulated with GM-CSF/IFN-γ/IL-3 and the expression of HLA-DR, CD80 and CD86 was assessed after 14 (white), 24 (light gray) and 48 h (dark gray); *P<0.05, Wilcoxon Signed Ranks test; (C) Lysates of freshly isolated neutrophils (0 h), monocytes (M), or neutrophils stimulated with GM-CSF/IFN-γ/IL-3 for 48 h sorted into HLA-DR+ and HLA-DR- cells were assessed for HLA-DM and GAPDH by immunoblotting;