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. Author manuscript; available in PMC: 2020 Jan 28.
Published in final edited form as: J Allergy Clin Immunol. 2018 Jun 18;143(3):1143–1152.e4. doi: 10.1016/j.jaci.2018.06.005

Figure 4. Detection of HLA-DR-positive neutrophils in cutaneous allergic LPR.

Figure 4

Allergen or diluent was injected intradermally and suction blisters were performed at the injection sites after 20-24 h. Fluids were analyzed for T-cells, monocytes, eosinophils and neutrophils by flow cytometry. Skin neutrophils were assessed for HLA-DR expression. One representative of five experiments is shown. In two patients, also blood neutrophils were isolated, cultured in medium or GM-CSF/IFN-γ/IL-3 for 24 h and analyzed for HLA-DR expression by flow cytometry.