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. Author manuscript; available in PMC: 2020 Jan 28.
Published in final edited form as: J Allergy Clin Immunol. 2018 Jun 18;143(3):1143–1152.e4. doi: 10.1016/j.jaci.2018.06.005

Figure E1. Expression of HLA-DR on cytokine-activated neutrophils.

Figure E1

(A) Purified neutrophils from six birch pollen-allergic individuals were cultured in medium alone (0) or stimulated with titrated concentrations 10(1), 1(2), 0.1(3), and 0.01(4) ng/ml) of GM-CSF, IFN-γ, and IL-3. The percentage of HLA-DR-positive cells was assessed by flow cytometry at 24 (white), 36 (light gray) and 48 h (dark gray box plots); (B) Neutrophils were left in medium alone (white) or stimulated with a cocktail of GM-CSF (100 pg/ml), IFN-γ (10 ng/ml) and IL-3 (30 ng/ml, gray) according to published protocols 1. The percentage of HLA-DR-positive cells was assessed at indicated time points; (C) The percentage of HLA-DR-positive cells expressing CD80 and CD86 at 24 h, the dot blot shows one representative experiment. *P<0.05, Wilcoxon Signed Ranks test;