Fig. 3.

a Rate of oleate oxidation in MCF7, MDA-MB-468 and MDA-MB-231 cells after 48 h treatment with 2 mM metformin and/or 100 µM etomoxir (n = 3–4). b The effects of metformin (2 mM) and etomoxir (100 µM) on total TGFA accumulation in MCF7 and MDA-MB-468 cells (n = 4–6). c All six cell lines were treated with 2 mM metformin and/or 100μM etomoxir vs. control for 96 h in media supplemented with 25 mM glucose or with 20 mM galactose but no glucose and cell number measured (n = 6). d Accumulation of DNL-derived TGFA in MCF7 and MDA-MB-468 cells (n = 3). e Percentage of TGFA synthesised via DNL as estimated from ²H₂O enrichment of fatty acid (n = 3). f Accumulation of TGFA in MCF7 cells derived from three different ¹³C-labelled substrates: glucose, glutamine and lactate (n = 3). Data shown are mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.