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Microscopic images of S. pistillata larvae from the shallow reef (A-C) and from the mesophotic reef (D-F). Green fluorescence indicates GFPs fluorescence (B, E), red fluorescence indicates the chlorophyll fluorescence of Symbiodinium (C, F), overlap of the 2 channels (A, D). Microscope exposure and gain settings are identical in both shallow and mesophotic larvae; chlorophyll exposure is 20ms and gain 1, GFP exposure is 150ms and gain 1.1. Magnification: 10x. Scale bar: 500 μm.
