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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Leukoc Biol. 2019 Sep 1;107(2):175–183. doi: 10.1002/JLB.1HI0219-073RR

FIGURE 2. Blocking β2 TMD transmission of conformation change in response to IL-8 impairs chemokine-induced β2 integrin-dependent arrest of rolling leukocytes.

FIGURE 2

(A) Adhesive behavior of CXCR2-transfected differentiated HL-60 cells (HL60), HL60β2-KO cells, same cells reconstituted with β2 WT or β2-L697P on a substrate of P-Selectin/ICAM-1 with (+) or without (−) IL8 stimulation at a wall shear stress of 6 dyn/cm2. Cells were pre-differentiated with 1.3% DMSO for 5–7 d and then infused into the flow chamber. (B) cumulative rolling velocity histograms for these same cells at 6 dyn/cm2. (C) Like panel A, but at a wall shear stress of 2 dyn/cm2. The arrested cell number (black, mean ±SD, n = 5 each in A and C) were analyzed. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. P > 0.05 by 2-way ANOVA followed by Bonferroni posttest