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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Dev Biol. 2019 Oct 9;458(1):12–31. doi: 10.1016/j.ydbio.2019.10.005

Figure 2. Effects of iCRT14 and LiCl treatments on the size of the regenerating gut rudiment in vivo.

Figure 2.

Schematic diagrams depicting the experimental protocol used for iCRT14 (A) or LiCl (E) treatment in vivo. Red arrows indicate the time points when the putative Wnt pathway antagonist (iCRT14) or the presumed Wnt pathway agonist (LiCl) were injected intracoelomically, black arrows indicate when the BrdU was injected, and X when the animals were sacrificed. Representative histological cross sections of intestines and attached mesenteries showing the effect of iCRT14 (B) and LiCl (F) treatment on gut rudiment size compared to the controls DMSO (C) and NaCl (G), respectively. Treatment with iCRT14 leads to a significant reduction in the gut rudiment area compared to controls (D). Conversely, treatment with LiCl results in a significant increase in the gut rudiment area compared to controls (H). Results represent the mean ± SD, t-test, *p<0.05, n= 9 iCRT14, n = 9 DMSO, n = 8 LiCl, n = 9 NaCl. Scale bars = 300 μm.