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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Dev Biol. 2019 Oct 9;458(1):12–31. doi: 10.1016/j.ydbio.2019.10.005

Fig. 6. Neither iCRT14 nor LiCl show any effect on apoptosis during intestine regeneration.

Fig. 6.

Programmed cell death was measured using TUNEL. Representative histological cross sections of gut rudiments showing the effect of iCRT14 (A,C) and LiCl (F,H) treatments on programmed cell death compared to controls DMSO (B,D) and NaCl (G,I), respectively. No significant differences in the percentage of TUNEL labeled cells were found between iCRT14-treated or LiCl-treated animals and their respective controls DMSO and NaCl (E and J, respectively). Results represent the mean ± SD, t-test, n = 8 iCRT14, n = 9 DMSO, n = 5 LiCl, n = 3 NaCl. The micrographs on the bottom (C,D,H,I) show the TUNEL labeled nuclei in white. Micrographs on the top (A,B,F,G) show the cell nuclei in red (DAPI) and TUNEL labeled nuclei (white arrows) in green. Scale bar = 50 μm.