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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Dev Biol. 2019 Oct 9;458(1):12–31. doi: 10.1016/j.ydbio.2019.10.005

Fig. 9. Neither iCRT14 nor LiCl alter apoptosis in vitro.

Fig. 9.

Programmed cell death was evaluated using TUNEL. Representative histological cross sections of gut explants showing the effect of iCRT14 (A,C) and LiCl (F,H) treatments on cell proliferation compared to controls DMSO (B,D) and NaCl (G,I), respectively. No significant differences in the percentage of TUNEL labeled nuclei were found between iCRT14-treated or LiCl-treated explants and their respective controls DMSO and NaCl (E and J, respectively). Results represent the mean ± SD, t-test, n = 4 iCRT14, n = 4 DMSO, n = 5 LiCl, n = 3 NaCl. The micrographs on the bottom (C,D,H,I) show TUNEL labeled nuclei (white arrows) in white. Micrographs on the top (A,B,F,G) show the cell nuclei in red (DAPI) and TUNEL labeled nuclei (white arrows) in green. Scale bar = 50 μm.