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. 2019 Oct 24;179(2):325–335. doi: 10.1007/s10549-019-05471-x

Fig. 2.

Fig. 2

a F11R/JAM-A (CD321) cell surface expression in non-tumorigenic MECs MCF-10A (black color) or in breast cancer cell lines (MCF-7, red color, and MDA-MB-231, green color) detected by flow cytometry. RT-PCR (b) and Western blot (c) assay for the detection of F11R/JAM-A expression levels using the RNA and protein extracts from HMEC-1 cells non-treated (C; control cells) or treated for 24 h with Tβ4 at a concentration of 200 nM or with TGF-β1 at a concentration of 10 ng/ml. RT-PCR (d) and Western blot (e) assay for the detection of F11R/JAM-A expression levels using the RNA and protein extracts from HMEC-1 cells incubated with Tβ4 at a concentration of 200 nM for 0, 2, 5, or 12 h. Figure presents the representative results of 3 independent experiments