Figure 1.

In silico construct of pIRSATeGFP_LUC. (A) Luciferase and eGFP genes cloned between the XbaI and BglII restriction sites, respectively. (B) Schematic representation of the SSU integration locus and the cassette for integration with eGFP and LUC ORF. (C) Schematic representation of the linear cassette for integration in the SSU rDNA locus. SSU, small subunit, DST IR, intergenic region of the DST gene, LUC, Luciferase, CYS2 IR, intergenic region of cysteine protease 2 gene, eGFP, enhanced GFP, LPG1R, intergenic region of the LPG1 gene, SAT, nourseothricine resistance gene, IR 1.7K, intergenic region of dihydrofolate reductase-thymidylate symthase locus. Position of the primers and size of amplified fragments are indicated by lines.