Figure 5.

In vitro infectivity of transgenic L. braziliensis co-expressing eGFP and Luciferase. Bone-marrow derived macrophages were infected with either WT or eGFP-LUC L. braziliensis (clone #7), for 4 h. Cells were processed for microscopy to assess the (A) percentage of infected cells and (B) the number of amastigotes per 200 macrophages Data (mean ± SEM) are from a representative experiment, performed in quintuplicate. (C) DIC, fluorescent (eGFP) and merged micrographs of macrophages infected with WT or eGFP-LUC L. braziliensis for 24 h.