Figure 1.

Screening of host dependency factors required by Toxoplasma gondii infection with CRISPR/CAS9-sgRNA library. (A) Screening flowchart. Human foreskin fibroblast (HFF) cells were transduced with lentiviruses at multiplicity of infection 1 (MOI = 1). Cells were subsequently selected using puromycin for 5 days. Transduced cells were infected with T. gondii (MOI = 1) for 10 days. Finally, the transduced cells that survived T. gondii infection were harvested, and the genomic DNA was extracted and subjected to high-throughput sequencing. (B) Venn diagram depicting the number of screened targets from independent biological triplicates: first replicate (red), second replicate (lime), third replicate (blue), and overlapped targets (middle).