Table 1.
Primers and adapters used in Proximity RNA-seq.
| Modification | Sequence (5′-3′) | Purification | |
|---|---|---|---|
| Bead barcoding | |||
| Primer-F | CCATCTCATCCCTGCGTGTC | HPLC | |
| Primer-R | CCTATCCCCTGTGTGCCTTG | HPLC | |
| Primer-R | 5′ dual biotin | CCACTACGCTCGCTATCCTATCCCCTGTGTGCCTTG | HPLC |
| Random barcode template | CCATCTCATCCCTGCGTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNGATCGTCGGACTGTAGAACTCCCTATAGTGAGTCGTATTACAAGGCACACAGGGGATAGG | PAGE | |
| Random tail primer F | NNNNNNNNNNNNNNNCCATCTCATCCCTGCGTGTC | HPLC | |
| Bead quality control (FACS) | |||
| T7 probe | 5′ Cy5 | CGTCGGACTGTAGAACTCCCTATAGTGAGTCGTA | HPLC |
| Library preparation/amplification | |||
| PolyC12 primer | GCCTTGGCACCCGAGAATTCCACCCCCCCCCCCC | PAGE | |
| RP1 | AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA | PAGE | |
| Index 6 | CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA | PAGE | |
| Index 12 | CAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA | PAGE | |
| Index 5 | CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA | PAGE | |
| Index 19 | CAAGCAGAAGACGGCATACGAGATTTTCACGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA | PAGE | |