Fig. 3. Fractional nutrient contribution (FNC) of glucose and acetate exposed to GYY4137.

a FNC was calculated according to a previous study³³; see also Materials and Methods. b, c Glycolysis is stimulated by sulfide as is evident by an increased FNC_glucose of G/F6P that corresponds with increased GYY4137 concentration, whereas the FNC_acetate and FNC_other decreases. The FNC_glucose of G-3-P (c) and S-7-P (e) increases with higher concentrations of GYY4137. d, f The utilization of glucose is skewed towards cell wall synthesis and the PPP leading to a decrease in FNC_glucose and FNC_acetate of succinate and malate as GYY4137 concentration increases, as well as a corresponding increase in FNC_other of these two metabolites. Data are representative of two independent experiments (n = 5) and are shown as mean ± SD. Data were analyzed using one-way ANOVA with Sidak’s multiple comparisons test. All P values are relative to untreated controls (0 µM GYY4137) for individual metabolites. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Source data are provided as a Source Data file.