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. 2020 Jan 28;10:1364. doi: 10.1038/s41598-020-58415-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Degree of melanisation of the beads injected into adult Anopheles gambiae according to larval treatments. The mosquitoes were inculated 4 days after emergence, and beads were recovered 24 h after the injection. The figure shows the amount of melanin deposited on beads (unmelanised beads are excluded). A grey value of 0 would mean that the bead is unmelanised, while a grey value of 256 means that the bead is black and thus completely melanised. The two pictures on the left give examples of an unmelanised (bottom left) and melanized (top left) bead. The sample sizes are, from left to right: N = 95, N = 107, N = 96, and N = 101. Error bars show the 95% confidence intervals of the means.