Figure 3.

Effect of suppression of NF-κB activation by JSH-23 on the production of E₂ and expression of steroidogenic genes in HESCs. (A) Concentrations of E₂ in the supernatant of HESCs (n = 8) treated with vehicle, activated platelets and activated platelets + JSH-23. (B) Gene expression levels of the genes involved in estrogen biosynthesis StAR, HSD3B2, CYP19A1 and HSD17B1 in HESCs co-cultured with vehicle, activated platelets and activated platelets + JSH-23. (C) Representative Western blotting results for StAR, HSD3B2, aromatase and HSD17B1 protein expression. (D) Summary of fold changes of protein expression levels of StAR, HSD3B2, aromatase and HSD17B1 in HESCs (n = 8) treated with vehicle, activated platelets and activated platelets + JSH-23. Summary of fold changes for SF-1 (E) and p-CREB (F) protein expression in HESCs (n = 8) treated with vehicle, activated platelets and activated platelets + JSH-23. “*” denotes that the p-value of the difference between the groups is less than 0.05. NS: p > 0.05. Paired Wilcoxon’s test was used.