Table 1.

TIC frequency of CaSki P3-no insert, CaSki P3-shHSP90AA1 #1, and CaSki P3-shHSP90AA1 #2 cells^a.

CaSki P3	Number of tumors/number of injected animalsb			TIC frequency (95% CI)c
	105	104	103
No insert	6/6	6/6	4/6	1:910 (1/2545–1/326)
shHSP90AA1 #1	4/6	2/6	0/6	1:68,078* (1/166,908–1/27,768)
shHSP90AA1 #2	3/6	2/6	0/6	1:96,052** (1/249,831–1/36,929)

TIC tumor-initiating cell, CI confidence interval

*p = 4.6 × 10⁻¹⁰ and **p = 2.35 × 10⁻¹¹ by pairwise tests

^aTo estimate tumor initiating properties of these cells, in vivo limiting dilution transplantation assay was performed

^bTumor cells of indicated number were subcutaneously injected into NOD/SCID mice (n = 6) and mice were scored for the presence of palpable tumors

^cTIC frequency and 95% CI were calculated by using ELDA software program (http://bioinf.wehi.edu.au/software/elda/)