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. 2020 Jan 28;10:1310. doi: 10.1038/s41598-020-58211-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Long-term stimulation of CD95 induces upregulation of endogenous retroviruses. (A) Real time PCR analysis of mRNAs of STAT1, PLSCR1, RIG-I, MDA5, and IRF7 of Cas9 control (wt, clone C1) and STAT1 k.o. MCF-7 cells (clone S1–6) after 0, 1, 2, 3 or 4 days treatment with LzCD95L. Error bars represent the SD of three biological replicates. Student’s t-test was performed to compare gene expression between 0 and 1 days treatment in wt samples. (B) Real time PCR analysis of selected ERVs MER21C, MLT1C49, MER4D, and ERVL of Cas9 control (wt, clone C1) and STAT1 k.o. cells (clone S1–6) after 0, 1, 2, 3 or 4 days treatment with LzCD95L. Error bars represent the SD of three biological replicates. Student’s t-test was performed to compare gene expression between 0 and 1 days treatment in wt samples. (C) Real time PCR analysis of selected ERVs in two Cas9 control clones (C1 and C2) and two caspase-8 k.o. clones (C8–11 and C8–17) after 4 days treatment with LzCD95L. Error bars represent the SD of three biological replicates. Student’s t-test was performed to compare gene expression between the average of LzCD95L treated control and two caspase-8 k.o. clones. (D) Real time PCR analysis of selected ERVs of MCF-7 cells after 4 days or treatment with different concentrations of IFNβ. Error bars represent the SD of four biological replicates. p-value *<0.05, **<0.001; ***<0.0001; ns, not significant.