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. 2020 Jan 28;10:1290. doi: 10.1038/s41598-020-57940-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cucurbitacin B and I induces G₂/M cell cycle arrest. (A) Cell cycle analysis of C-B and C-I treated cells. HCT116 and SW480 cells were treated with up to 5 μM C-B and C-I for 48 h and examined by flow cytometry following propidium iodide staining for DNA content. C-B and C-I treatment induced cell cycle arrest at the G₂/M phase of the cell cycle. (B) Lysates from HCT116 and SW480 cells treated with 5 μM of C-B and C-I for 24 h and 48 h were analyzed by western blotting for the expression of proteins involved in the regulation of cell cycle. CDK2-Cyclin A2 and CDC2-Cyclin B1 are significantly downregulated after compounds treatment indicates G₂/M arrest of the cell cycle.