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. 2020 Jan 28;10:1290. doi: 10.1038/s41598-020-57940-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cucurbitacin B and I induces apoptosis. (A) HCT116 and SW480 cells were treated with 5 μM of C-B and C-I for 48 h, stained with Annexin V (FITC) and PI, and analyzed by flow cytometry. C-B and C-I treatment induced significant early and late apoptosis in HCT116 and SW480 cells. (B) Lysates from HCT116 and SW480 cells treated with C-B and C-I induced significant cleavage of PARP and Caspase 3 as compared to untreated controls. The treatment also increased the expression of pro-apoptotic marker Bax and reduced anti-apoptotic marker Bcl-XL, Mcl-1 and Bcl-2 as compared to untreated controls. (C) Caspase 3/7 assay demonstrates an increase in the caspase activity in HCT116 and SW480 cells 48 h post-treatment with C-B and C-I (*p < 0.01).