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. 2020 Jan 28;9:11. doi: 10.1038/s41377-020-0249-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — The regions enclosed by the dashed yellow boxes on the left are enlarged on the right. a–f Representative examples of the colocalization of DBs with Rab5a-EGFP- (a), EEA1-EGFP- (b), FYVE-EGFP- (c), Rab9a-EGFP- (d), Rab7-EGFP- (e), LAMP1-EGFP- (f), and AQP2-EGFP- (g) labeled vesicles. h Left: proportions of DBs associated with fluorescent Rab5a/EEA1/FYVE/Rab9a/Rab7/LAMP1 within the total pool of DBs are shown in orange, while those without fluorescence labeling are shown in gray. Right: proportions of DBs associated with Rab5a/EEA1/FYVE/Rab9a/Rab7/LAMP1 within the total pools of the respective fluorescent vesicles are shown in orange, while those of non-DB fluorescent vesicles within the total pools of the respective fluorescent vesicles are shown in gray. i Left: diameters of DBs with (orange) and without (gray) fluorescent labeling and fluorescent vesicles with visible ODT structures except DBs (blue). Right: differences in RIs between cellular structures and their surrounding environments, which include DBs with (orange) and without (gray) fluorescent labeling and fluorescent vesicles with visible ODT structures, except DBs (blue). Scale bars, (a–g) 5 μm (left) and 1 μm (right).