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. 2020 Jan 28;10:1383. doi: 10.1038/s41598-020-58395-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of OsHSP20 in rice seedlings following various stresses: temperature of 45 °C (heat stress) (A), 100 mM NaCl (salinity stress) (B), 5% PEG (drought stress) (C), or 100 μM ABA (hormone stress) (D) analyzed by qRT-PCR (A–D) and Western blotting (E,F), in which the exposure time is 20 s or 150 s for detection of HSP20 protein under heat or salt stress, respectively. Data are mean ± SD from three independent experiments. The asterisks on the top of the columns indicate significant differences from the value at 0 h (ns, not significant; *P < 0.05; **P < 0.01).