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. 2020 Jan 28;10:1383. doi: 10.1038/s41598-020-58395-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of root lengths (A,B) and seed germination rates (C) of transgenic OsHSP20 rice plants under salt stress. (A) Root length phenotype of 2-week-old untransformed wild-type (WT) and OsHSP20 overexpression lines (Ubi:OsHSP20-L5, -L16 and -L20). WT and transgenic plants were germinated on 1/2 MS medium plates for 3 days before transfer to a new MS medium plate supplemented with 100 mM NaCl for 2 weeks. Scale bar is 1 cm. (B) Quantitative analysis of primary root lengths of 2-week-old seedlings from WT and each transgenic line. The average (±SD) values are from three biological replicates with 50 plants for each line and replicate. Significant differences between WT and transgenic plants are indicated by asterisks (ns, not significant; **P < 0.01). (C) Germination rates were measured at 6 d after incubating seeds at 25 °C with or without salt stress. Data represent the mean ± SD (P < 0.05) from three independent experiments.