Figure 2.

The cGMP enhancer vardenafil reduces active Rab5 levels. N2a cells were treated with 100 μM vardenafil (VDF) for different times (5, 10, 30, 60 min). Control samples (0 min) received the same volume of vehicle (DMSO) for 60 min. At the end of treatments, cells were processed for total protein extraction followed by Rab5 activity assay, as described in the Methods section. The image displays cropped blots. Graphed data show mean ± SEM for four independent experiments (*P < 0.05; **P < 0.005 vs the vehicle-treated group).