Figure 1.

Samples of DNA from individual plants of event GR2E in Kaybonnet (Tn; lanes 7–8, as replicates), BRRI dhan 29 (BC₃F₅, lanes 9–10; BC₄F₃, lanes 15–16; and BC₅F₃, lanes 21–22), IR64 (BC₃F₅, lanes 11–12; BC₄F₃, lanes 17–18; and BC₅F₃, lanes 23–24), and PSB Rc82 (BC₃F₅, lanes 13–14; BC₄F₃, lanes 19–20; and BC₅F₃, lanes 25–26) germplasm backgrounds and negative control DNA from Kaybonnet rice (lanes 5–6) were subjected to Southern blot analysis. For this, 5 μg genomic DNA was digested with HindIII (panel A), SphI (panel B), or AscI plus XmaI (panel C) followed by agarose gel electrophoresis and transfer onto nylon membrane. Positive control samples consisted of negative control Kaybonnet rice containing either one (lane 3) or 0.2 (lane 4) copy equivalents of pSYN12424 plasmid DNA digested with SphI (panels A and B) or AscI + XmaI (panel C), Blots were hybridized with DIG-labelled probes for Zmpsy1 (panel A), pSSU-crtI (panel B), or pmi (panel C). Following washing, hybridized probes and DIG-labelled molecular weight markers VII (lanes 1 and 28) were visualized using a chemiluminescent detection. Lanes 2 and 27 were blank on all gels (adapted from GR2E-FFP submitted study reports).