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. 2020 Jan 28;10:1297. doi: 10.1038/s41598-020-58187-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Standardization of modified ARMS PCR assay from the genomic DNA of Trichophyton mentagrophytes complex and Trichophyton rubrum; Lane 1 = Trichophyton rubrum (NCCPF 900041) with higher MICs to terbinafine having T1189C mutation; Lane 2, 3, 4 = Trichophyton mentagrophytes complex (NCCPF 800035, 800036, 800038) with low MICs to terbinafine. Lane 5, 6 = Trichophyton mentagrophytes complex (NCCPF 800022, 800023) with higher MIC’s to terbinafine having T1189C mutation; Lane 7,10,11 Trichophyton mentagrophytes complex (NCCPF 800062, 800063, 800064) with higher MICs to terbinafine having C1191A mutation; Lane 8, 9 = Trichophyton mentagrophytes complex (NCCPF 800035, 800036) with low MICs to terbinafine. Lane 1–6 were amplified using mutation primer-1 and lane 7–11 amplified using mutation primer-2. (M = 100 base pair ladder). (B) Validation of the modified ARMS PCR assay against genomic DNA of representative T. mentagrophytes complex with higher MICs to terbinafine. Lane 1, 2, 3, 4, 6 = Trichophyton mentagrophytes complex (NCCPF 800062, 800063, 800064, 800065, 800066) with high terbinafine MIC’s and C1191A mutation; lane 5, 7 = Trichophyton mentagrophytes complex (NCCPF 800043, 800044) with low MICs to terbinafine; Lane 8, 9, 10, 12, 13, 14, 16 = Trichophyton mentagrophytes complex (NCCPF 800055, 800056, 800052, 800053, 800060, 800061, 800057) with T1189C mutation; Lane 11, 15, = Trichophyton mentagrophytes complex (NCCPF 800043, 800044) with low terbinafine MICs. Lane 1–7 were amplified using mutation primer-2 and Lane 8–16 amplified with mutation primer-1. (M = 100 base pair ladder).