Table 2.

Strains and plasmids used in this study.

Strains	Genotype/Description
DH10B (recA−)	F−endA1 recA1 galE15 galK16 nupGrpsLΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrAΔ(mrr-hsdRMS-mcrBC) λ−
DH5α	F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi–1 gyrA96 relA1
RosettaTM (DE3)	E. coli strain used for protein expression; F− ompT hsdSB (rB− mB−) gal-dcm (DE3) pRARE (CamR)
Plasmids
pBC SK	Cloning vector, Camr
pCR-Blunt II-TOPO	Cloning vector, Kanr
pHis-parallel1	Vector for in vitro expression with T7 promoter, adds GB1 solubility tag; IPTG inducible; restriction enzyme cloning; Ampr